Bi-specific and Multivalent Formats / Formatting

Palomo C, Mas V, Detalle L, Depla E, Cano O, Vázquez M, Stortelers C, Melero JA. Trivalency of a Nanobody specific for the human respiratory syncytial virus fusion glycoprotein drastically enhances virus neutralization and impacts escape mutant selection. Antimicrob Agents Chemother. 2016 Nov; 60:11: 6498-6509.

Vosjan MJWD, Vercammen J, Kolkman JA, Stigter-van Walsum M, Revets H, van Dongen GAMS. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy. Mol Cancer Ther. 2012;11:1017-1025.

Rajabi-Memari H, Jalali-Javaran M, Rasaee MJ, Rahbarizadeh F, Forouzandeh-Moghadam M, Esmaili A. Expression and characterization of a recombinant single-domain monoclonal antibody against MUC1 Mucin in tobacco plants. Hybridoma (Larchmt). 2006 Aug; 25 (4): 209-15.

Lewis W, Keshavarz-Moore E, Windust J, Bushell D, Parry N.  Construction and evaluation of novel fusion proteins for targeted delivery of micro particles to cellulose surfaces.  Biotechnol Bioeng. 2006 Jul 5; 94 (4): 625-32.

Coppieters K, Dreier T, Silence K, Haard HD, Lauwereys M, Casteels P, Beirnaert E, Jonckheere H, Wiele CV, Staelens L, Hostens J, Revets H, Remaut E, Elewaut D, Rottiers P.  Formatted anti-tumor necrosis factor alpha VHH proteins derived from camelids show superior potency and targeting to inflamed joints in a murine model of collagen-induced arthritis.  Arthritis Rheum. 2006 Jun; 54 (6): 1856-66.

Szynol A, de Haard JJ, Veerman EC, de Soet JJ, van Nieuw Amerogen AV.  Design of a peptibody consisting of the antimicrobial peptide dhvar5 and a llama variable heavy-chain fragment.  Chem Biol Drug Des. 2006 Jun; 67 (6): 425-31.

Baral TN, Magez S, Stijlemans B, Conrath K, Vanhollebeke B, Pays E, Muyldermans S, De Baetselier P.  Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factor.  Nat Med. 2006 May; 12 (5): 580-4.

Saerens D, Frederix F, Reekmans G, Conrath K, Jans K, Brys L, Huang L, Bosmans E, Maes G, Borghs G, Muyldermans S.  Engineering camel single-domain antibodies and immobilization chemistry for human prostate-specific antigen sensing.  Anal Chem. 2005 Dec 1; 77 (23): 7547-55.

Zou X, Smith JA, Nguyen VK, Ren L, Luyten K, Muyldermans S, Bruggemann M.  Expression of a dromedary heavy chain-only antibody and B cell development in the mouse.  J Immunol. 2005 Sep 15; 175 (6): 3769-79.

Huang L, Reekmans G, Saerens D, Friedt JM, Frederix F, Francis L, Muyldermans S, Campitelli A, Van Hoof C.  Prostate-specific antigen immunosensing based on mixed self-assembled monolayers, camel antibodies and colloidal gold enhanced sandwich assays.  Biosens Bioelectron. 2005 Sep 15; 21(3):483-90.

Gibbs WW.  Nanobodies.  Sci Am. 2005 Aug; 293 (2): 78-83.

Szynol A, de Soet JJ, Sieben-van Tuyl E, Bos JW, Frenken LG.  Bactericidal effects of a fusion protein of llama heavy-chain antibodies coupled to glucose oxidase on oral bacteria.  Antimicrob Agents Chemother. 2004 Sep; 48 (9): 3390-5.

Zhang J, Li Q, Nguyen TD, Tremblay TL, Stone E, To R, Kelly J, Roger MacKenzie C.  A pentavalent single-domain antibody approach to tumor antigen discovery and the development of novel proteomics reagents.  J Mol Biol. 2004 Jul 30; 341 (1): 161-9.

Pleschberger M, Saerens D, Weigert S, Sleytr UB, Muyldermans S, Sara M, Egelseer EM.  An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology.  Bioconjug Chem. 2004 May-Jun; 15 (3): 664-71.

Cortez-Retamozo V, Backmann N, Senter PD, Wernery U, De Baetselier P, Muyldermans S, Revets H.  Efficient cancer therapy with a nanobody-based conjugate.  Cancer Res. 2004 Apr 15; 64 (8): 2853-7.

Zhang J, Tanha J, Hirama T, Khieu NH, To R, Tong-Sevinc H, Stone E, Brisson JR, MacKenzie CR.  Pentamerization of single-domain antibodies from phage libraries: a novel strategy for the rapid generation of high-avidity antibody reagents.  J Mol Biol. 2004 Jan 2; 335 (1): 49-56.

Nguyen VK, Zou X, Lauwereys M, Brys L, Bruggemann M, Muyldermans S.  Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells.  Immunology. 2003 May; 109 (1): 93-101.

Pleschberger M, Neubauer A, Egelseer EM, Weigert S, Lindner B, Sleytr UB, Muyldermans S, Sara M.  Generation of a functional monomolecular protein lattice consisting of an S-layer fusion protein comprising the variable domain of a camel heavy chain antibody.  Bioconjug Chem. 2003 Mar-Apr; 14 (2): 440-8.

Joosten V, Lokman C, Van Den Hondel CA, Punt PJ.  The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi.  Microb Cell Fact. 2003 Jan 30; 2 (1): 1.

Muyldermans S.  Single domain camel antibodies: current status.  J Biotechnol. 2001 Jun; 74 (4): 277-302.

Conrath KE, Lauwereys M, Wyns L, Muyldermans S.  Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.  J Biol Chem. 2001 Mar 9; 276 (10): 7346-50.

 < back