Stability

Omidfar K, Rasaee MJ, Kashanian S, Paknejad M, Bathaie Z. Studies of thermostability in Camelus bactrianus (Bactrian camel) single-domain antibody specific for the mutant epidermal-growth-factor receptor expressed by Pichia. Biotechnol Appl Biochem. 2007 Jan; 46 (Pt1): 41.

Pant N, Hultberg A, Zhao Y, Svensson L, Pan-Hammarstrom Q, Johansen K, Pouwels PH, Ruggeri FM, Hermans P, Frenken L, Boren T, Marcotte H, Hammarstrom L. Lactobacili expressing variable domain of llama heavy-chain antibody fragments (lactobodies) confer protection against rotavirus-induced diarrhea. J Infect Dis. 2006 Dec 1; 194 (11): 1580-8.

Ladenson RC, Crimmins DL, Landt Y, Ladenson JH.  Isolation and characterization of a thermally stable recombinant anti-caffeine heavy-chain antibody fragment.  Anal Chem. 2006 Jul 1; 78 (13): 4501-8.

Kruger C, Hultberg A, Marcotte H, Hermans P, Bezemer S, Frenken LG, Hammarstrom L.  Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries.  Appl Microbiol Biotechnol. 2006 Apr 25.

Harmsen MM, van Solt CB, van Zijderveld-van Bemmel AM, Niewold TA, van Zijderveld FG.  Selection and optimization of proteolytically stable llama single-domain antibody fragments for oral immunotherapy.  Appl Microbiol Biotechnol. 2006 Feb 1; 1-8.

Verheesen P, de Kluijver A, van Koningsbruggen S, de Brij M, de Haard HJ, van Ommen GJ, van der Maarel SM, Verrips CT.  Prevention of oculopharyngeal muscular dystrophy-associated aggregation of nuclear polyA-binding protein with a single-domain intracellular antibody.  Hum Mol Genet. 2006 Jan 1; 15 (1): 105-11.

Gibbs WW.  Nanobodies.  Sci Am. 2005 Aug; 293 (2): 78-83.

Conrath K, Vincke C, Stijlemans B, Schymkowitz J, Decanniere K, Wyns L, Muyldermans S, Loris R.  Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH.  J Mol Biol. 2005 Jul 1; 350 (1): 112-25.

Dolk E, van Vliet C, Perez JM, Vriend G, Darbon H, Ferrat G, Cambillau C, Frenken LG, Verrips T.  Induced refolding of a temperature denatured llama heavy-chain antibody fragment by its antigen.  Proteins. 2005 May 15; 59 (3): 555-64.

Dolk E, van der Vaart M, Lutje Hulsik D, Vriend G, de Haard H, Spinelli S, Cambillau C, Frenken L, Verrips T.  Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo.  Appl Environ Microbiol. 2005 Jan; 71(1): 442-50.

Verheesen P, Ten Haaft MR, Lindner N, Verrips CT, de Haard JJ.  Beneficial properties of single-domain antibody fragments for application in immunoaffinity purification and immuno-perfusion chromatography.  Biochim Biophys Acta 2003 Dec 5; 1624 (1-3): 21-8.

Dekker S, Toussaint W, Panayotou G, de Wit T, Visser P, Grosveld F, Drabek D.  Intracellularly expressed single-domain antibody against p15 matrix protein prevents the production of porcine retroviruses.  J Virol. 2003 Nov; 77 (22): 12132-9.

Dumoulin M, Last AM, Desmyter A, Decanniere K, Canet D, Larsson G, Spencer A, Archer DB, Sasse J, Muyldermans S, Wyns L, Redfield C, Matagne A, Robinson CV, Dobson CM.  A camelid antibody fragment inhibits the formation of amyloid fibrils by human lysozyme.  Nature. 2003 Aug 14; 424 (6950): 783-8.

Jobling SA, Jarman C, Teh MM, Holmberg N, Blake C, Verhoeyen ME.  Immunomodulation of enzyme function in plants by single-domain antibody fragments.  Nat Biotechnol. 2003 Jan; 21 (1): 77-80.

Dumoulin M, Conrath K, Van Meirhaeghe A, Meersman F, Heremans K, Frenken LG, Muyldermans S, Wyns L, Matagne A.  Single-domain antibody fragments with high conformational stability.  Protein Sci. 2002 Mar; 11 (3): 500-15.

Muyldermans S.  Single domain camel antibodies: current status.  J Biotechnol. 2001 Jun; 74 (4): 277-302.

Perez JM, Renisio JG, Prompers JJ, van Platerink CJ, Cambillau C, Darbon H, Frenken LG.  Thermal unfolding of a llama antibody fragment: a two-state reversible process.  Biochemistry 2001 Jan 9; 40(1): 74-83.

Van der Linden RH, Frenken LG, de Geus B, Harmsen MM, Ruuls RC, Stok W, de Ron L, Wilson S, Davis P, Verrips CT.  Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies.  Biochim Biophys Acta 1999 Apr 12; 1431(1): 37-46.

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