Production Features

Schotte P, Dewerte I, De Groeve M, De Keyser S, De Brabandere V, Stanssens P. Pichia pastoris Mut(S) strains are prone to misincorporation of O-methyl-L-homoserine at methionine residues when methanol is used as the sole carbon source. Microb Cell Fact. 2016 Jun 7;15(1):98.

Pant N, Hultberg A, Zhao Y, Svensson L, Pan-Hammarstrom Q, Johansen K, Pouwels PH, Ruggeri FM, Hermans P, Frenken L, Boren T, Marcotte H, Hammarstrom L. Lactobacili expressing variable domain of llama heavy-chain antibody fragments (lactobodies) confer protection against rotavirus-induced diarrhea. J Infect Dis. 2006 Dec 1; 194 (11): 1580-8

Ismaili A, Jalali-Javaran M, Rasaee MJ, Rahbarizadeh F, Forouzandeh-Moghadam M, Memari HR. Production and characterization of anti-(mucin MUC1) single-domain antibody in tobacco. Biotechnol Appl Biochem. 2006 Oct 30

Zhong Y, Zhang XH, Chen J, Chi Z, Sun B, Li Y, Austin B. Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH. Infect Immun. 2006 Oct; 74 (10): 6001-5

Rahbarizadeh F, Rasaee MJ, Forouzandeh M, Allameh AA.  Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastorisMol Immunol. 2006 Feb; 43 (5): 426-35.

Arbabi-Ghahroudi M, Tanha J, MacKenzie R.  Prokaryotic expression of antibodies.  Cancer Metastasis Rev. 2005 Dec; 24 (4): 501-19.

Rahbarizadeh F, Rasaee MJ, Forouzandeh-Moghadam M, Allameh AA.  High expression and purification of the recombinant camelid anti-MUC1 single domain antibodies in Escherichia coliProtein Expr Purif. 2005 Nov; 44(1): 32-8.

Gibbs WW.  Nanobodies.  Sci Am. 2005 Aug; 293 (2): 78-83.

Joosten V, Gouka RJ, van den Hondel CA, Verrips CT, Lokman BC.  Expression and production of llama variable heavy-chain antibody fragments (V(HH)s) by Aspergillus awamoriAppl Microbiol Biotechnol. 2005 Jan; 66 (4): 384-92.

Cortez-Retamozo V, Backmann N, Senter PD, Wernery U, De Baetselier P, Muyldermans S, Revets H.  Efficient cancer therapy with a nanobody-based conjugate.  Cancer Res. 2004 Apr 15; 64 (8): 2853-7.

Nguyen VK, Zou X, Lauwereys M, Brys L, Bruggemann M, Muyldermans S.  Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells.  Immunology. 2003 May; 109 (1): 93-101.

Joosten V, Lokman C, Van Den Hondel CA, Punt PJ.  The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi.  Microb Cell Fact. 2003 Jan 30; 2 (1): 1.

Ledeboer AM, Bezemer S, de Haard JJ, Schaffers IM, Verrips CT, van Vliet C, Düsterhöft EM, Zoon P, Moineau S, Frenken LG. Preventing phage lysis of Lactococcus lactis in cheese production using a neutralizing heavy-chain antibody fragment from llama. J Dairy Sci. 2002 Jun; 85(6): 1376-82.

van der Vaart JM.  Expression of VHH antibody fragments in Saccharomyces cerevisiaeMethods Mol Biol. 2002; 178: 359-66.

Muyldermans S.  Single domain camel antibodies: current status.  J Biotechnol. 2001 Jun; 74 (4): 277-302.

van der Linden RH, de Geus B, Frenken GJ, Peters H, Verrips CT. Improved production and function of llama heavy chain antibody fragments by molecular evolution. J Biotechnol. 2000 Jul 14;80(3):261-70.

Frenken LG, van der Linden RH, Hermans PW, Bos JW, Ruuls RC, de Geus B, Verrips CT. Isolation of antigen specific llama VHH antibody fragments and their high level secretion by Saccharomyces cerevisiae. J Biotechnol. 2000 Feb 28;78(1):11-21.

Frenken LG, Hessing JG, Van den Hondel CA, Verrips CT. Recent advances in the large-scale production of antibody fragments using lower eukaryotic microorganisms. Res Immunol. 1998 Jul-Aug;149(6):589-99.

Arbabi Ghahroudi M, Desmyter A, Wyns L, Hamers R, Muyldermans S.  Selection and identification of single domain antibody fragments from camel heavy-chain antibodies.  FEBS Lett. 1997 Sep 15; 414(3): 521-6.

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