Three-dimensional structure of a Nanobody® (modeled on the VHH with pdb-code 1U0Q, Cambillau et al). The target molecule is recognized by the surface formed by three hypervariable regions located at the top of the molecule (in green, cyan and blue). Hallmarks, amino acid residues that further differentiate a camelid VHH from a human VH, can be located at different positions within the framework regions, and here are coloured red, pink and magenta.

Home R & D Nanobody® Bibliography > High Selectivity

Nanobody® Bibliography

High Selectivity

Lauwereys M, Ghahroudi MA, Desmyter A, Kinne J, Hölzer W, De Genst E, Wyns L, Muyldermans S.  Potent enzyme inhibitors derived from dromedary heavy-chain antibodies.  EMBO J. 1998 Jul 1; 17(13): 3512-20.

Muyldermans S.  Single domain camel antibodies: current status.  J Biotechnol. 2001 Jun; 74 (4): 277-302.

Cortez-Retamozo V, Backmann N, Senter PD, Wernery U, De Baetselier P, Muyldermans S, Revets H.  Efficient cancer therapy with a nanobody-based conjugate.  Cancer Res. 2004 Apr 15; 64 (8): 2853-7.

Saerens D, Kinne J, Bosmans E, Wernery U, Muyldermans S, Conrath K.  Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen.  J Biol Chem. 2004 Dec 10; 279 (50): 51965-72.

Gibbs WW.  Nanobodies.  Sci Am. 2005 Aug; 293 (2): 78-83.

Huang L, Reekmans G, Saerens D, Friedt JM, Frederix F, Francis L, Muyldermans S, Campitelli A, Van Hoof C.  Prostate-specific antigen immunosensing based on mixed self-assembled monolayers, camel antibodies and colloidal gold enhanced sandwich assays.  Biosens Bioelectron. 2005 Sep 15; 21(3):483-90.

Hulstein JJ, de Groot PG, Silence K, Veyradier A, Fijnheer R, Lenting PJ.  A novel nanobody that detects the gain-of-function phenotype of von Willebrand factor in ADAMTS13 deficiency and von Willebrand disease type 2B.  Blood. 2005 Nov 1; 106 (9): 3035-42.