Gemakkelijke en goedkope productie
Arbabi Ghahroudi M, Desmyter A, Wyns L, Hamers R, Muyldermans S. Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett. 1997 Sep 15; 414(3): 521-6.
Frenken LG, Hessing JG, Van den Hondel CA, Verrips CT. Recent advances in the large-scale production of antibody fragments using lower eukaryotic microorganisms. Res Immunol. 1998 Jul-Aug;149(6):589-99.
Frenken LG, van der Linden RH, Hermans PW, Bos JW, Ruuls RC, de Geus B, Verrips CT. Isolation of antigen specific llama VHH antibody fragments and their high level secretion by Saccharomyces cerevisiae. J Biotechnol. 2000 Feb 28; 78(1): 11-21.
van der Linden RH, de Geus B, Frenken GJ, Peters H, Verrips CT. Improved production and function of llama heavy chain antibody fragments by molecular evolution. J Biotechnol. 2000 Jul 14; 80(3): 261-70.
Muyldermans S. Single domain camel antibodies: current status. J Biotechnol. 2001 Jun; 74 (4): 277-302.
van der Vaart JM. Expression of VHH antibody fragments in Saccharomyces cerevisiae. Methods Mol Biol. 2002; 178: 359-66.
Ledeboer AM, Bezemer S, de Haard JJ, Schaffers IM, Verrips CT, van Vliet C, Dusterhoft EM, Zoon P, Moineau S, Frenken LG. Preventing phage lysis of Lactococcus lactis in cheese production using a neutralizing heavy-chain antibody fragment from llama. J Dairy Sci. 2002 Jun; 85 (6): 1376-82.
Thomassen YE, Meijer W, Sierkstra L, Verrips CT. Large-scale production of VHH antibody fragments by Saccharomyces cerevisiae. Enzyme and Microbial Technol. 2002; 30: 273-8.
Nguyen VK, Zou X, Lauwereys M, Brys L, Bruggemann M, Muyldermans S. Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells. Immunology. 2003 May; 109 (1): 93-101.
Joosten V, Lokman C, Van Den Hondel CA, Punt PJ. The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. Microb Cell Fact. 2003 Jan 30; 2 (1): 1.
Cortez-Retamozo V, Backmann N, Senter PD, Wernery U, De Baetselier P, Muyldermans S, Revets H. Efficient cancer therapy with a nanobody-based conjugate. Cancer Res. 2004 Apr 15; 64 (8): 2853-7.
Joosten V, Gouka RJ, van den Hondel CA, Verrips CT, Lokman BC. Expression and production of llama variable heavy-chain antibody fragments (V(HH)s) by Aspergillus awamori. Appl Microbiol Biotechnol. 2005 Jan; 66 (4): 384-92.
Gibbs WW. Nanobodies. Sci Am. 2005 Aug; 293 (2): 78-83.
Thomassen YE, Verkleij AJ, Boonstra J, Verrips CT. Specific production rate of VHH antibody fragments by Saccharomyces cerevisiae is correlated with growth rate, independent of nutrient limitation. J Biotechnol. 2005 Aug 22; 118 (3): 270-7.
Rahbarizadeh F, Rasaee MJ, Forouzandeh-Moghadam M, Allameh AA. High expression and purification of the recombinant camelid anti-MUC1 single domain antibodies in Escherichia coli. Protein Expr Purif. 2005 Nov; 44(1): 32-8.
Arbabi-Ghahroudi M, Tanha J, MacKenzie R. Prokaryotic expression of antibodies. Cancer Metastasis Rev. 2005 Dec; 24 (4): 501-19.
Rahbarizadeh F, Rasaee MJ, Forouzandeh M, Allameh AA. Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris. Mol Immunol. 2006 Feb; 43 (5): 426-35.
Zhong Y, Zhang XH, Chen J, Chi Z, Sun B, Li Y, Austin B. Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH. Infect Immun. 2006 Oct; 74 (10): 6001-5
Ismaili A, Jalali-Javaran M, Rasaee MJ, Rahbarizadeh F, Forouzandeh-Moghadam M, Rajabi Memari H. Production and Characterization of anti-MUC1 single-domain antibody in tobacco. Biotechnol Appl Biochem. 2006 Oct 30
Pant N, Hultberg A, Zhao Y, Svensson L, Pan-Hammarstrom Q, Johansen K, Pouwels PH, Ruggeri FM, Hermans P, Frenken L, Boren T, Marcotte H, Hammarstrom L. Lactobacili expressing variable domain of llama heavy-chain antibody fragments (lactobodies) confer protection against rotavirus-induced diarrhea. J Infect Dis. 2006 Dec 1; 194 (11): 1580-8